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rat multiple tissue northern blot  (TaKaRa)


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    TaKaRa rat multiple tissue northern blot
    ( A ) <t>Northern</t> <t>blot</t> analysis of <t>rat</t> iap mRNA expression in adult rat tissues. A rat <t>multiple</t> <t>tissue</t> northern blot (Clontech) containing 2 μg/lane poly(A) + RNA per lane was probed sequentially with [ 32 P] dCTP (Amersham) labeled, random primed (Amersham Rediprime) DNA probes derived from the coding regions of rat iap-1 , iap-2 , iap-3, and β-actin (control). Blots were hybridized overnight in 5 × SSPE/10 × Denhardt's solution/100 μg/ml salmon sperm DNA/ 50% formamide/ 2% SDS and then washed with 0.2 × SSC/ 0.1% SDS at 50°C. The position and sizes of the markers are indicated on the left. The tissues represented on the blot are as follows: ( 1 ) heart, ( 2 ) brain, ( 3 ) spleen, ( 4 ) lung, ( 5 ) liver, ( 6 ) skeletal muscle, ( 7 ) kidney, and ( 8 ) testis. ( B ) Northern blot analysis of the expression of iap -2 in testes. A rat multiple tissue Northern blot (Clontech; same as in (A)) was hybridized sequentially with [ 32 P] dCTP labeled DNA probes specific to iap -2 coding region, BIR domain and the RING zing finger. The blots were processed as in (A). The position of testis-specific transcript is indicated by an asterisk ( * ).
    Rat Multiple Tissue Northern Blot, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 96 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rat+multiple+tissue+northern+blot/pmc00065541-85-1-6?v=TaKaRa
    Average 90 stars, based on 96 article reviews
    rat multiple tissue northern blot - by Bioz Stars, 2026-07
    90/100 stars

    Images

    1) Product Images from "Cloning and characterization of the rat homologues of the Inhibitor of Apoptosis protein 1, 2, and 3 genes."

    Article Title: Cloning and characterization of the rat homologues of the Inhibitor of Apoptosis protein 1, 2, and 3 genes.

    Journal: BMC Genomics

    doi: 10.1186/1471-2164-3-5

    ( A ) Northern blot analysis of rat iap mRNA expression in adult rat tissues. A rat multiple tissue northern blot (Clontech) containing 2 μg/lane poly(A) + RNA per lane was probed sequentially with [ 32 P] dCTP (Amersham) labeled, random primed (Amersham Rediprime) DNA probes derived from the coding regions of rat iap-1 , iap-2 , iap-3, and β-actin (control). Blots were hybridized overnight in 5 × SSPE/10 × Denhardt's solution/100 μg/ml salmon sperm DNA/ 50% formamide/ 2% SDS and then washed with 0.2 × SSC/ 0.1% SDS at 50°C. The position and sizes of the markers are indicated on the left. The tissues represented on the blot are as follows: ( 1 ) heart, ( 2 ) brain, ( 3 ) spleen, ( 4 ) lung, ( 5 ) liver, ( 6 ) skeletal muscle, ( 7 ) kidney, and ( 8 ) testis. ( B ) Northern blot analysis of the expression of iap -2 in testes. A rat multiple tissue Northern blot (Clontech; same as in (A)) was hybridized sequentially with [ 32 P] dCTP labeled DNA probes specific to iap -2 coding region, BIR domain and the RING zing finger. The blots were processed as in (A). The position of testis-specific transcript is indicated by an asterisk ( * ).
    Figure Legend Snippet: ( A ) Northern blot analysis of rat iap mRNA expression in adult rat tissues. A rat multiple tissue northern blot (Clontech) containing 2 μg/lane poly(A) + RNA per lane was probed sequentially with [ 32 P] dCTP (Amersham) labeled, random primed (Amersham Rediprime) DNA probes derived from the coding regions of rat iap-1 , iap-2 , iap-3, and β-actin (control). Blots were hybridized overnight in 5 × SSPE/10 × Denhardt's solution/100 μg/ml salmon sperm DNA/ 50% formamide/ 2% SDS and then washed with 0.2 × SSC/ 0.1% SDS at 50°C. The position and sizes of the markers are indicated on the left. The tissues represented on the blot are as follows: ( 1 ) heart, ( 2 ) brain, ( 3 ) spleen, ( 4 ) lung, ( 5 ) liver, ( 6 ) skeletal muscle, ( 7 ) kidney, and ( 8 ) testis. ( B ) Northern blot analysis of the expression of iap -2 in testes. A rat multiple tissue Northern blot (Clontech; same as in (A)) was hybridized sequentially with [ 32 P] dCTP labeled DNA probes specific to iap -2 coding region, BIR domain and the RING zing finger. The blots were processed as in (A). The position of testis-specific transcript is indicated by an asterisk ( * ).

    Techniques Used: Northern Blot, Expressing, Labeling, Random Primed, Derivative Assay

    The relative expression levels of the rat iap genes in various tissues as detected by Northern or Western blot analysis.
    Figure Legend Snippet: The relative expression levels of the rat iap genes in various tissues as detected by Northern or Western blot analysis.

    Techniques Used: Expressing, Northern Blot, Western Blot



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    rat multiple tissue northern blot  (TaKaRa)
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    TaKaRa rat multiple tissue northern blot
    ( A ) <t>Northern</t> <t>blot</t> analysis of <t>rat</t> iap mRNA expression in adult rat tissues. A rat <t>multiple</t> <t>tissue</t> northern blot (Clontech) containing 2 μg/lane poly(A) + RNA per lane was probed sequentially with [ 32 P] dCTP (Amersham) labeled, random primed (Amersham Rediprime) DNA probes derived from the coding regions of rat iap-1 , iap-2 , iap-3, and β-actin (control). Blots were hybridized overnight in 5 × SSPE/10 × Denhardt's solution/100 μg/ml salmon sperm DNA/ 50% formamide/ 2% SDS and then washed with 0.2 × SSC/ 0.1% SDS at 50°C. The position and sizes of the markers are indicated on the left. The tissues represented on the blot are as follows: ( 1 ) heart, ( 2 ) brain, ( 3 ) spleen, ( 4 ) lung, ( 5 ) liver, ( 6 ) skeletal muscle, ( 7 ) kidney, and ( 8 ) testis. ( B ) Northern blot analysis of the expression of iap -2 in testes. A rat multiple tissue Northern blot (Clontech; same as in (A)) was hybridized sequentially with [ 32 P] dCTP labeled DNA probes specific to iap -2 coding region, BIR domain and the RING zing finger. The blots were processed as in (A). The position of testis-specific transcript is indicated by an asterisk ( * ).
    Rat Multiple Tissue Northern Blot, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rat+multiple+tissue+northern+blot/pmc00065541-85-1-6?v=TaKaRa
    Average 90 stars, based on 1 article reviews
    rat multiple tissue northern blot - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier
    rat multiple-tissue northern blot  (OriGene)
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    OriGene rat multiple-tissue northern blot
    ( A ) <t>Northern</t> <t>blot</t> analysis of <t>rat</t> iap mRNA expression in adult rat tissues. A rat <t>multiple</t> <t>tissue</t> northern blot (Clontech) containing 2 μg/lane poly(A) + RNA per lane was probed sequentially with [ 32 P] dCTP (Amersham) labeled, random primed (Amersham Rediprime) DNA probes derived from the coding regions of rat iap-1 , iap-2 , iap-3, and β-actin (control). Blots were hybridized overnight in 5 × SSPE/10 × Denhardt's solution/100 μg/ml salmon sperm DNA/ 50% formamide/ 2% SDS and then washed with 0.2 × SSC/ 0.1% SDS at 50°C. The position and sizes of the markers are indicated on the left. The tissues represented on the blot are as follows: ( 1 ) heart, ( 2 ) brain, ( 3 ) spleen, ( 4 ) lung, ( 5 ) liver, ( 6 ) skeletal muscle, ( 7 ) kidney, and ( 8 ) testis. ( B ) Northern blot analysis of the expression of iap -2 in testes. A rat multiple tissue Northern blot (Clontech; same as in (A)) was hybridized sequentially with [ 32 P] dCTP labeled DNA probes specific to iap -2 coding region, BIR domain and the RING zing finger. The blots were processed as in (A). The position of testis-specific transcript is indicated by an asterisk ( * ).
    Rat Multiple Tissue Northern Blot, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rat+multiple+tissue+northern+blot/pmc03873836-89-3-7?v=OriGene
    Average 90 stars, based on 1 article reviews
    rat multiple-tissue northern blot - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    ( A ) Northern blot analysis of rat iap mRNA expression in adult rat tissues. A rat multiple tissue northern blot (Clontech) containing 2 μg/lane poly(A) + RNA per lane was probed sequentially with [ 32 P] dCTP (Amersham) labeled, random primed (Amersham Rediprime) DNA probes derived from the coding regions of rat iap-1 , iap-2 , iap-3, and β-actin (control). Blots were hybridized overnight in 5 × SSPE/10 × Denhardt's solution/100 μg/ml salmon sperm DNA/ 50% formamide/ 2% SDS and then washed with 0.2 × SSC/ 0.1% SDS at 50°C. The position and sizes of the markers are indicated on the left. The tissues represented on the blot are as follows: ( 1 ) heart, ( 2 ) brain, ( 3 ) spleen, ( 4 ) lung, ( 5 ) liver, ( 6 ) skeletal muscle, ( 7 ) kidney, and ( 8 ) testis. ( B ) Northern blot analysis of the expression of iap -2 in testes. A rat multiple tissue Northern blot (Clontech; same as in (A)) was hybridized sequentially with [ 32 P] dCTP labeled DNA probes specific to iap -2 coding region, BIR domain and the RING zing finger. The blots were processed as in (A). The position of testis-specific transcript is indicated by an asterisk ( * ).

    Journal: BMC Genomics

    Article Title: Cloning and characterization of the rat homologues of the Inhibitor of Apoptosis protein 1, 2, and 3 genes.

    doi: 10.1186/1471-2164-3-5

    Figure Lengend Snippet: ( A ) Northern blot analysis of rat iap mRNA expression in adult rat tissues. A rat multiple tissue northern blot (Clontech) containing 2 μg/lane poly(A) + RNA per lane was probed sequentially with [ 32 P] dCTP (Amersham) labeled, random primed (Amersham Rediprime) DNA probes derived from the coding regions of rat iap-1 , iap-2 , iap-3, and β-actin (control). Blots were hybridized overnight in 5 × SSPE/10 × Denhardt's solution/100 μg/ml salmon sperm DNA/ 50% formamide/ 2% SDS and then washed with 0.2 × SSC/ 0.1% SDS at 50°C. The position and sizes of the markers are indicated on the left. The tissues represented on the blot are as follows: ( 1 ) heart, ( 2 ) brain, ( 3 ) spleen, ( 4 ) lung, ( 5 ) liver, ( 6 ) skeletal muscle, ( 7 ) kidney, and ( 8 ) testis. ( B ) Northern blot analysis of the expression of iap -2 in testes. A rat multiple tissue Northern blot (Clontech; same as in (A)) was hybridized sequentially with [ 32 P] dCTP labeled DNA probes specific to iap -2 coding region, BIR domain and the RING zing finger. The blots were processed as in (A). The position of testis-specific transcript is indicated by an asterisk ( * ).

    Article Snippet: A rat multiple tissue northern blot (Clontech) containing 2 μg/lane poly(A) + RNA per lane was probed sequentially with [ 32 P] dCTP (Amersham) labeled, random primed (Amersham Rediprime) DNA probes derived from the coding regions of rat iap-1 , iap-2 , and iap-3, or β-actin, respectively .

    Techniques: Northern Blot, Expressing, Labeling, Random Primed, Derivative Assay

    The relative expression levels of the rat iap genes in various tissues as detected by Northern or Western blot analysis.

    Journal: BMC Genomics

    Article Title: Cloning and characterization of the rat homologues of the Inhibitor of Apoptosis protein 1, 2, and 3 genes.

    doi: 10.1186/1471-2164-3-5

    Figure Lengend Snippet: The relative expression levels of the rat iap genes in various tissues as detected by Northern or Western blot analysis.

    Article Snippet: A rat multiple tissue northern blot (Clontech) containing 2 μg/lane poly(A) + RNA per lane was probed sequentially with [ 32 P] dCTP (Amersham) labeled, random primed (Amersham Rediprime) DNA probes derived from the coding regions of rat iap-1 , iap-2 , and iap-3, or β-actin, respectively .

    Techniques: Expressing, Northern Blot, Western Blot